Suppelementary videos for "Allometric cell spreading and the geometrical control of focal adhesion collective organization"

Biophys J, 2025

  1. Time-lapse of Ref-52 fibroblast expressing paxilin-YFP spreading on a bi-dimensional glass substrate, imaged via TIRF microscopy. Time is in min:sec. Scale bar: 5um.
  2. Time-lapse of Ref-52 fibroblast expressing paxilin-YFP spreading between two parallel glass plate. Bottom cell-substrate contact is imaged via TIRF microscopy. Time is in min:sec. Scale bar: 5um.
  3. Time-lapse of Ref-52 fibroblast expressing paxilin-YFP treated with mitomycin C spreading on a bi-dimensional glass substrate, imaged via TIRF microscopy. Time is in min:sec. Scale bar: 5um.
  4. Time-lapse of Ref-52 fibroblast expressing paxilin-YFP treated with Y27632 at 8µM spreading on a bi-dimensional glass substrate, imaged via TIRF microscopy. Time is in min:sec. Scale bar: 5um.
  5. Time-lapse of Ref-52 fibroblast spreading on a glass plate seen in profile, imaged via bright-field microscopy. Time is in min:sec. Scale bar: 5um.
  6. Time-lapse of Ref-52 fibroblast spreading between parallel glass plate seen in profile, imaged via bright-field microscopy. Time is in min:sec. Scale bar: 5um.
  7. Segmentation and markers of cell profile along spreading of a Ref-52 fibroblast spreading on a glass plate. Scale bar: 5um.
  8. Shape changes and markers of the profile of a spherical cap of constant volume, mimicking cell spreading on a bi-dimensional substrate.
  9. Shape changes and markers of the profile of a constant mean curvature surface in contact with two parallel planes, mimicking cell spreading between parallel plates.
  10. Three-dimensional representation of shape changes of a constant mean curvature surface in contact with two parallel planes, mimicking cell spreading between parallel plates.
  11. Time-lapse of Ref-52 fibroblast expressing paxilin-YFP spreading on a bi-dimensional glass substrate, imaged via TIRF microscopy, showing two steps of spreading and two successive focal adhesions ring forming. Time is in min:sec. Scale bar: 5um.

  • SM01Pax-YFP_1plate_10-11-25-m4_dt=30_scale5um_5fps.avi (1.7M, RIFF (little-endian) data, AVI, 1107 x 933, 5.00 fps, video: Motion JPEG)

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  • SM02Pax-YFP_2plates_12-01-24-m1_dt=45s_scale5um_5fps.avi (1.5M, RIFF (little-endian) data, AVI, 938 x 880, 5.00 fps, video: Motion JPEG)

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  • SM03Pax-YFP_MMC_12-07-18-m1_dt=30s_scale5um_5fps.avi (2.4M, RIFF (little-endian) data, AVI, 1108 x 1038, 5.00 fps, video: Motion JPEG)

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  • SM04Pax-YFP_Y27632_11-12-20-m3_dt=30s_scale5um_5fps.avi (3.7M, RIFF (little-endian) data, AVI, 1032 x 954, 5.00 fps, video: Motion JPEG)

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  • SM05Profile_1plate_20-06-04-m1-b_dt=10_scale5um_20fps.avi (1016K, RIFF (little-endian) data, AVI, 663 x 355, 20.00 fps, video: Motion JPEG)

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  • SM06Profile_2plates_12-04-11-m4-scale5um-20fps.avi (1.7M, RIFF (little-endian) data, AVI, 571 x 309, 20.00 fps, video: Motion JPEG)

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  • SM07Profile-Analysis_20-06-01-m3-a.avi (9.8M, RIFF (little-endian) data, AVI, 2742 x 2466, 5.00 fps, video: Motion JPEG)

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  • SM08Simu-1plate-_4fps.avi (3.4M, RIFF (little-endian) data, AVI, 2560 x 1920, 4.00 fps, video: Motion JPEG)

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  • SM09Simu-2plates-d=095_4fps.avi (2.5M, RIFF (little-endian) data, AVI, 2126 x 1364, 4.00 fps, video: Motion JPEG)

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  • SM10-Simu-2plates-3D-d=095_4fps.avi (3.6M, RIFF (little-endian) data, AVI, 1959 x 1971, 4.00 fps, video: Motion JPEG)

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  • SM11-Pax-YFP_DoubleSpread_10-11-23-m2_dt=40_scale5um_5fps.avi (2.2M, RIFF (little-endian) data, AVI, 1107 x 1038, 5.00 fps, video: Motion JPEG)