The QuanTI-FRET has been reworked to allow calibration with FRET standards or autocalibration directly for the images of interest! A software is being prepared as standalone in Python and plug-in for Napari.
QuanTI-FRET Git
General scheme of the QuanTI-FRET method.
More details in "QuanTI-FRET: a framework for quantitative FRET measurements in living cells." A Coullomb, CM Bidan, C Qian, F Wehnekamp, C Oddou, C Albiges-Rizo, DC Lamb et A Dupont. Scientific Reports 10 (1), 1-11 (2020)